immunochemical characterization of prosopis juliflora pollen allergens and evaluation of cross-reactivity pattern with the most allergenic pollens in tropical areas.
نویسندگان
چکیده
allergy to prosopis juliflora (mesquite) pollen is one of the common causes of respiratory allergy in tropical countries. mesquite is widely used as street trees in towns and ornamental shade trees in parks and gardens throughout arid and semiarid regions of iran. the inhalation of mesquite pollen and several species of amaranthus/chenopodiaceae family is the most important cause of allergic respiratory symptoms in khuzestan province. this study was designed to evaluate ige banding proteins of mesquite pollen extract and its ige cross-reactivity with other allergenic plants. twenty patients with allergic symptoms and positive skin prick tests (spt) for mesquite pollen extract participated in the study. crude pollen extract was prepared from local mesquite trees and used for the evaluation of allergenic profiles of p. juliflora pollen extract by sodium dodecylsulfate polyacrylamide gel electrophoresis (sds-page) and ige-immunoblotting. there were several protein bands in mesquite pollen extract using sds-page with the approximate range of molecular weight of 10-85 kda. the most frequent ige reactive bands among the patients' sera were approximately 20 and 66 kda. however, there were other ige reactive protein bands among the patients' sera with molecular weights of 10, 15, 35, 45, 55 and 85 kda. inhibition experiments revealed high ige cross-reactivity between mesquite and acacia. there are several ige-binding proteins in p. juliflora pollen extract. results of this study indicate that proteins with a molecular weight of 10 to 85 kda are the major allergens in p. juliflora pollen extract.
منابع مشابه
Immunochemical characterization of prosopis juliflora pollen allergens and evaluation of cross-reactivity pattern with the most allergenic pollens in tropical areas.
Allergy to Prosopis juliflora (mesquite) pollen is one of the common causes of respiratory allergy in tropical countries. Mesquite is widely used as street trees in towns and ornamental shade trees in parks and gardens throughout arid and semiarid regions of Iran. The inhalation of mesquite pollen and several species of Amaranthus/Chenopodiaceae family is the most important cause of allergic re...
متن کاملImmunochemical Characterization of Acacia Pollen Allergens and Evaluation of Cross-Reactivity Pattern with the Common Allergenic Pollens
Pollen from the Acacia has been reported as an important source of pollinosis in tropical and subtropical regions of the world. The aim of this study was to characterize the IgE binding protein of Acacia farnesiana pollen extract and evaluate cross-reactivity with the most allergenic pollens. In this study, pollen extract was fractionated by SDS-PAGE and the allergenic profile was determined by...
متن کاملThe human allergens of mesquite (Prosopis juliflora)
BACKGROUND: A computerized statistical analysis of allergy skin test results correlating patient reactivities initiated our interest in the cross-reactive allergens of mesquite tree pollen. In-vitro testing with mesquite-sensitized rabbits and a variety of deciduous tree pollens revealed so many cross-reactivities that it became apparent there could be more allergens in mesquite than previously...
متن کاملPectate Lyase Pollen Allergens: Sensitization Profiles and Cross-Reactivity Pattern
BACKGROUND Pollen released by allergenic members of the botanically unrelated families of Asteraceae and Cupressaceae represent potent elicitors of respiratory allergies in regions where these plants are present. As main allergen sources the Asteraceae species ragweed and mugwort, as well as the Cupressaceae species, cypress, mountain cedar, and Japanese cedar have been identified. The major al...
متن کاملthe evaluation of language related engagment and task related engagment with the purpose of investigating the effect of metatalk and task typology
abstract while task-based instruction is considered as the most effective way to learn a language in the related literature, it is oversimplified on various grounds. different variables may affect how students are engaged with not only the language but also with the task itself. the present study was conducted to investigate language and task related engagement on the basis of the task typolog...
15 صفحه اولمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of allergy, asthma and immunologyجلد ۱۴، شماره ۱، صفحات ۷۴-۸۲
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023